2. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (2106):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0821
    CY5 146368-11-8 99.66%
    CY5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    CY5
  • HY-D0822
    CY3 146368-13-0 ≥98.0%
    Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
    CY3
  • HY-D0017
    Dansyl chloride 605-65-2 ≥98.0%
    Dansyl chloride is a reagent that produces stable blue or blue-green fluorescent sulfonamide adducts in the reaction of aliphatic and aromatic amines with primary amino groups, and is widely used for modified amino acids, protein sequencing and amino acid analysis.
    Dansyl chloride
  • HY-D0826
    Cy2-SE (iodine) 186205-33-4
    Cy2-SE iodine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy2-SE (iodine)
  • HY-D0827
    Cy2 260430-02-2 ≥98.0%
    Cyanines are formally compounds with two nitrogen atoms linked by an odd number of methene units. 26 28 The nitrogen atoms are parts of the heterocyclic units (such as indole, benzoxazol, or benzothiazol) . The structures and optical properties of representative cyanine dyes used for in vivo imaging are presented. Cyanines are characterized by long wavelength, tunable absorption and emission, very high extinction coefficient (up to 300,000 M 1 cm 1), good water solubility, and relatively straightforward synthesis.
    Cy2
  • HY-D0715
    Fluorescamine 38183-12-9 ≥98.0%
    Fluorescamine( Ro 20-7234) is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products.
    Fluorescamine
  • HY-125750
    Coumarin 7 27425-55-4 99.10%
    Coumarin 7 is a coumarin laser dye in plants in the form of glycosides.
    Coumarin 7
  • HY-130533
    ReAsH-EDT2 438226-89-2
    ReAsH-EDT2 is a red fluorescent dye that marks proteins. ReAsH-EDT2 is a membrane-permeable biarsenical compound that binds covalently to tetracysteine sequences which allows the protein to be imaged. ReAsH-EDT2 can be used for protein localization and trafficking. (λex=530 nm, λem=592 nm).
    ReAsH-EDT2
  • HY-101890
    Dabsyl chloride 56512-49-3 ≥98.0%
    Dabsyl chloride is an amine derivatizing agent, able to give rise to stable products that can be easily monitored spectrophotometrically at 460 nm; Dabsyl chloride also used for labeling amino acids.
    Dabsyl chloride
  • HY-D0818
    CY3-YNE 1010386-62-5
    CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. CY3-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    CY3-YNE
  • HY-D0820
    CY5-YNE 1345823-20-2
    CY5-YNE (Sulfo-Cyanine5-alkyne) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. CY5-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    CY5-YNE
  • HY-Y0700
    Calconcarboxylic acid 3737-95-9
    Calconcarboxylic acid, an azo dye, acts as a silver-ion sensitizer to stain protein in SDS-PAGE gels. Calconcarboxylic acid increases silver binding on protein bands or spots by the formation of a silver-dye complex and also increases the reducing power o
    Calconcarboxylic acid
  • HY-145383
    Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 2599839-59-3
    Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide is a clickable, acid-cleavable biotin-picolyl azide. Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide is an enrichment handle of cell surface glycoproteins for protein labeling.
    Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide
  • HY-D0968
    Cy 3 (Non-Sulfonated) 146397-20-8
    Cy 3 Non-Sulfonated (Cyanine3) is a fluorescent label for protein and nucleic acid.
    Cy 3 (Non-Sulfonated)
  • HY-D1403
    L-Biotin-NH-5MP 2100288-18-2
    L-Biotin-NH-5MP is a biotin-conjugated 5-Methylene pyrrolone (5MP). 5-Methylene pyrrolones (5MPs) are thiol-specific, reversible bioconjugation reagents for cysteine-specific protein modification, that are commonly used for protein bioconjugation.
    L-Biotin-NH-5MP
  • HY-D1404
    5MP-Propargyl 2100286-15-3
    5MP-Propargyl is the derivative of 5-Methylene pyrrolone (5MP). 5-Methylene pyrrolones (5MPs) are thiol-specific, reversible bioconjugation reagents for cysteine-specific protein modification, that are commonly used for protein bioconjugation. 5MP-Propargyl is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    5MP-Propargyl
  • HY-D1405
    Br-5MP-Fluorescein 2468100-39-0
    Br-5MP-Fluorescein is a dye reagent for labeling of peptides and proteins.
    Br-5MP-Fluorescein
  • HY-D1406
    L-Biotin-NH-5MP-Br 2468100-38-9
    L-Biotin-NH-5MP-Br is a biotin-conjugated 5-Methylene pyrrolone (5MP). 5-Methylene pyrrolones (5MPs) are thiol-specific, reversible bioconjugation reagents for cysteine-specific protein modification, that are commonly used for protein bioconjugation.
    L-Biotin-NH-5MP-Br
  • HY-D1407
    Br-5MP-Propargyl 2468100-37-8
    Br-5MP-Propargyl is the derivative of 5-Methylene pyrrolone (5MP). 5-Methylene pyrrolones (5MPs) are thiol-specific, reversible bioconjugation reagents for cysteine-specific protein modification, that are commonly used for protein bioconjugation. Br-5MP-Propargyl is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    Br-5MP-Propargyl
  • HY-D1607
    BODIPY FL SSE 217190-17-5
    BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm).
    BODIPY FL SSE